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Marcella Sarzotti-Kelsoe, PhD

Research Professor of Immunology
Campus Mail: 2812 Erwin Rd., Suite 301, Durham, NC 27705
Phone: (919) 684-6373
Email: marcella.kelsoe@duke.edu

Ongoing Applied Activities 
•I direct a Global Quality Assurance Program, which I developed and pioneered here at Duke University, to oversee compliance with Good Clinical Laboratory Practice Guidelines in three HIV vaccine trial networks (CHAVI, CAVD, Duke HVTN, EQAPOL, Duke VTEU) involving domestic and international laboratory sites.
•I also direct a Global Proficiency Testing Program for laboratories testing for neutralizing antibody function in individuals infected with HIV or vaccinated against HIV. The Program was launched in 2009.
•I provide assistance and oversight for endpoint assay standardization, qualification and validation, as well as for the QSU of the GMP facility at DHVI, which will manufacture HIV vaccine products for first-in-man Phase I trials.

Past Basic Research
•Development of T cell responses in neonates.
•Neonatal T cell receptor Vβ repertoire diversity in the peripheral T cell pool.
•The role of heat shock protein, as a natural adjuvant, at eliciting innate and adaptive immune responses.
•Development of the T cell receptor repertoire in naïve, immunodeficient infants, given bone marrow or thymic transplantation.
•Thymic output, T cell diversity and T cell function in long-term human SCID chimeras.
•Telomere length in T cells from SCID chimeras.

Education and Training

  • D.R., University of Turin (Italy), 1986
  • Ph.D., University of Turin (Italy), 1980

Selected Publications

Sarzotti-Kelsoe, Marcella, Chan M. Win, Roberta E. Parrott, Myriah Cooney, Barry K. Moser, Joseph L. Roberts, Gregory D. Sempowski, and Rebecca H. Buckley. “Thymic output, T-cell diversity, and T-cell function in long-term human SCID chimeras.” Blood 114, no. 7 (August 13, 2009): 1445–53. https://doi.org/10.1182/blood-2009-01-199323.

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Sarzotti-Kelsoe, Marcella, Josephine Cox, Naana Cleland, Thomas Denny, John Hural, Leila Needham, Daniel Ozaki, et al. “Evaluation and recommendations on good clinical laboratory practice guidelines for phase I-III clinical trials.” Plos Med 6, no. 5 (May 26, 2009): e1000067. https://doi.org/10.1371/journal.pmed.1000067.

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Markert, M Louise, Blythe H. Devlin, Marilyn J. Alexieff, Jie Li, Elizabeth A. McCarthy, Stephanie E. Gupton, Ivan K. Chinn, et al. “Review of 54 patients with complete DiGeorge anomaly enrolled in protocols for thymus transplantation: outcome of 44 consecutive transplants.” Blood 109, no. 10 (May 15, 2007): 4539–47. https://doi.org/10.1182/blood-2006-10-048652.

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Roberts, Joseph L., Jens Peter H. Lauritsen, Myriah Cooney, Roberta E. Parrott, Elisa O. Sajaroff, Chan M. Win, Michael D. Keller, et al. “T-B+NK+ severe combined immunodeficiency caused by complete deficiency of the CD3zeta subunit of the T-cell antigen receptor complex.” Blood 109, no. 8 (April 15, 2007): 3198–3206. https://doi.org/10.1182/blood-2006-08-043166.

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Fadel, Shaza A., Lindsay G. Cowell, Shui Cao, Daniel A. Ozaki, Thomas B. Kepler, Douglas A. Steeber, and Marcella Sarzotti. “Neonate-primed CD8+ memory cells rival adult-primed memory cells in antigen-driven expansion and anti-viral protection.” Int Immunol 18, no. 2 (February 2006): 249–57. https://doi.org/10.1093/intimm/dxh360.

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Mascola, John R., Patricia D’Souza, Peter Gilbert, Beatrice H. Hahn, Nancy L. Haigwood, Lynn Morris, Christos J. Petropoulos, Victoria R. Polonis, Marcella Sarzotti, and David C. Montefiori. “Recommendations for the design and use of standard virus panels to assess neutralizing antibody responses elicited by candidate human immunodeficiency virus type 1 vaccines.” J Virol, August 2005. https://doi.org/10.1128/JVI.79.16.10103-10107.2005.

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Markert, M Louise, Marilyn J. Alexieff, Jie Li, Marcella Sarzotti, Daniel A. Ozaki, Blythe H. Devlin, Debra A. Sedlak, et al. “Postnatal thymus transplantation with immunosuppression as treatment for DiGeorge syndrome.” Blood 104, no. 8 (October 15, 2004): 2574–81. https://doi.org/10.1182/blood-2003-08-2984.

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Baker-LePain, Julie C., Marcella Sarzotti, and Christopher V. Nicchitta. “Glucose-regulated protein 94/glycoprotein 96 elicits bystander activation of CD4+ T cell Th1 cytokine production in vivo.” J Immunol 172, no. 7 (April 1, 2004): 4195–4203. https://doi.org/10.4049/jimmunol.172.7.4195.

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Markert, M Louise, Marcella Sarzotti, Daniel A. Ozaki, Gregory D. Sempowski, Maria E. Rhein, Laura P. Hale, Francoise Le Deist, et al. “Thymus transplantation in complete DiGeorge syndrome: immunologic and safety evaluations in 12 patients.” Blood 102, no. 3 (August 1, 2003): 1121–30. https://doi.org/10.1182/blood-2002-08-2545.

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Sarzotti, Marcella, Dhavalkumar D. Patel, Xiaojing Li, Daniel A. Ozaki, Shui Cao, Scott Langdon, Roberta E. Parrott, Katherine Coyne, and Rebecca H. Buckley. “T cell repertoire development in humans with SCID after nonablative allogeneic marrow transplantation.” J Immunol 170, no. 5 (March 1, 2003): 2711–18. https://doi.org/10.4049/jimmunol.170.5.2711.

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Baker-LePain, Julie C., Marcella Sarzotti, Timothy A. Fields, Chuan-Yuan Li, and Christopher V. Nicchitta. “GRP94 (gp96) and GRP94 N-terminal geldanamycin binding domain elicit tissue nonrestricted tumor suppression.” J Exp Med 196, no. 11 (December 2, 2002): 1447–59. https://doi.org/10.1084/jem.20020436.

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Fadel, Shaza A., Daniel A. Ozaki, and Marcella Sarzotti. “Enhanced type 1 immunity after secondary viral challenge in mice primed as neonates.” J Immunol 169, no. 6 (September 15, 2002): 3293–3300. https://doi.org/10.4049/jimmunol.169.6.3293.

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Sarzotti, M., T. A. Dean, M. P. Remington, C. D. Ly, P. A. Furth, and D. S. Robbins. “Induction of cytotoxic T cell responses in newborn mice by DNA immunization.” Vaccine 15, no. 8 (June 1997): 795–97. https://doi.org/10.1016/s0264-410x(96)00250-2.

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