Sarzotti-Kelsoe, M, Daniell, XG, Whitesides, JF, and Buckley, RH. "The long and the short of telomeres in bone marrow recipient SCID patients." Immunol Res 49, no. 1-3 (April 2011): 44-48.
Marcella Sarzotti-Kelsoe, PhD
Ongoing Applied Activities
•I direct a Global Quality Assurance Program, which I developed and pioneered here at Duke University, to oversee compliance with Good Clinical Laboratory Practice Guidelines in three HIV vaccine trial networks (CHAVI, CAVD, Duke HVTN, EQAPOL, Duke VTEU) involving domestic and international laboratory sites.
•I also direct a Global Proficiency Testing Program for laboratories testing for neutralizing antibody function in individuals infected with HIV or vaccinated against HIV. The Program was launched in 2009.
•I provide assistance and oversight for endpoint assay standardization, qualification and validation, as well as for the QSU of the GMP facility at DHVI, which will manufacture HIV vaccine products for first-in-man Phase I trials.
Past Basic Research
•Development of T cell responses in neonates.
•Neonatal T cell receptor Vβ repertoire diversity in the peripheral T cell pool.
•The role of heat shock protein, as a natural adjuvant, at eliciting innate and adaptive immune responses.
•Development of the T cell receptor repertoire in naïve, immunodeficient infants, given bone marrow or thymic transplantation.
•Thymic output, T cell diversity and T cell function in long-term human SCID chimeras.
•Telomere length in T cells from SCID chimeras.
Education and Training
- D.R., University of Turin (Italy), 1986
- Ph.D., University of Turin (Italy), 1980
Selected Grants and Awards
- Nonhuman Primate Core-Option 6
- EQAPOL Option 47
- EQAPOL - 2016 to 2017 - Option 6 - BASE
- EQAPOL - Option 48 - 2016 to 2017 - LUMINEX
- EQAPOL Option 5
- Animal Models of Infectious Diseases Part A
- HIV Vaccine Trials Network: LC P5 PF
- LC: HIV Vaccine Trials Network
- HIV Vaccine Trials Network: LC P5 PF
- LC: HIV Vaccine Trials Network: Phase 1 PF
- IQA Option 4
- Production of HIV-1 Envs #2
- Mechanisms of Allogeneic Stem Cell Education in SCID
- Role Of Surfactant In Innate and Adaptive Immunity
- Dose Study of Thymus Transplantation, IND 9836
- Thymic Transplantation in Complete DiGeorge Syndrome
- Memory T Cell Induction In Neonates By Viral Vaccines
Sarzotti-Kelsoe, M, Win, CM, Parrott, RE, Cooney, M, Moser, BK, Roberts, JL, Sempowski, GD, and Buckley, RH. "Thymic output, T-cell diversity, and T-cell function in long-term human SCID chimeras." Blood 114, no. 7 (August 13, 2009): 1445-1453.
Sarzotti-Kelsoe, M, Cox, J, Cleland, N, Denny, T, Hural, J, Needham, L, Ozaki, D, Rodriguez-Chavez, IR, Stevens, G, Stiles, T, Tarragona-Fiol, T, and Simkins, A. "Evaluation and recommendations on good clinical laboratory practice guidelines for phase I-III clinical trials." PLoS Med 6, no. 5 (May 26, 2009): e1000067-.
Markert, ML, Devlin, BH, Alexieff, MJ, Li, J, McCarthy, EA, Gupton, SE, Chinn, IK, Hale, LP, Kepler, TB, He, M, Sarzotti, M, Skinner, MA, Rice, HE, and Hoehner, JC. "Review of 54 patients with complete DiGeorge anomaly enrolled in protocols for thymus transplantation: outcome of 44 consecutive transplants." Blood 109, no. 10 (May 15, 2007): 4539-4547.
Roberts, JL, Lauritsen, JPH, Cooney, M, Parrott, RE, Sajaroff, EO, Win, CM, Keller, MD, Carpenter, JH, Carabana, J, Krangel, MS, Sarzotti, M, Zhong, X-P, Wiest, DL, and Buckley, RH. "T-B+NK+ severe combined immunodeficiency caused by complete deficiency of the CD3zeta subunit of the T-cell antigen receptor complex." Blood 109, no. 8 (April 15, 2007): 3198-3206.
Fadel, SA, Cowell, LG, Cao, S, Ozaki, DA, Kepler, TB, Steeber, DA, and Sarzotti, M. "Neonate-primed CD8+ memory cells rival adult-primed memory cells in antigen-driven expansion and anti-viral protection." Int Immunol 18, no. 2 (February 2006): 249-257.
Mascola, JR, D'Souza, P, Gilbert, P, Hahn, BH, Haigwood, NL, Morris, L, Petropoulos, CJ, Polonis, VR, Sarzotti, M, and Montefiori, DC. "Recommendations for the design and use of standard virus panels to assess neutralizing antibody responses elicited by candidate human immunodeficiency virus type 1 vaccines." J Virol 79, no. 16 (August 2005): 10103-10107.
Markert, ML, Alexieff, MJ, Li, J, Sarzotti, M, Ozaki, DA, Devlin, BH, Sedlak, DA, Sempowski, GD, Hale, LP, Rice, HE, Mahaffey, SM, and Skinner, MA. "Postnatal thymus transplantation with immunosuppression as treatment for DiGeorge syndrome." Blood 104, no. 8 (October 15, 2004): 2574-2581.
Baker-LePain, JC, Sarzotti, M, and Nicchitta, CV. "Glucose-regulated protein 94/glycoprotein 96 elicits bystander activation of CD4+ T cell Th1 cytokine production in vivo." J Immunol 172, no. 7 (April 1, 2004): 4195-4203.
Markert, ML, Sarzotti, M, Ozaki, DA, Sempowski, GD, Rhein, ME, Hale, LP, Le Deist, F, Alexieff, MJ, Li, J, Hauser, ER, Haynes, BF, Rice, HE, Skinner, MA, Mahaffey, SM, Jaggers, J, Stein, LD, and Mill, MR. "Thymus transplantation in complete DiGeorge syndrome: immunologic and safety evaluations in 12 patients." Blood 102, no. 3 (August 1, 2003): 1121-1130.
Sarzotti, M, Patel, DD, Li, X, Ozaki, DA, Cao, S, Langdon, S, Parrott, RE, Coyne, K, and Buckley, RH. "T cell repertoire development in humans with SCID after nonablative allogeneic marrow transplantation." J Immunol 170, no. 5 (March 1, 2003): 2711-2718.
Baker-LePain, JC, Sarzotti, M, Fields, TA, Li, C-Y, and Nicchitta, CV. "GRP94 (gp96) and GRP94 N-terminal geldanamycin binding domain elicit tissue nonrestricted tumor suppression." J Exp Med 196, no. 11 (December 2, 2002): 1447-1459.
Fadel, SA, Ozaki, DA, and Sarzotti, M. "Enhanced type 1 immunity after secondary viral challenge in mice primed as neonates." J Immunol 169, no. 6 (September 15, 2002): 3293-3300.
Cerasoli, DM, Kelsoe, G, and Sarzotti, M. "CD4+Thy1- thymocytes with a Th-type 2 cytokine response." Int Immunol 13, no. 1 (January 2001): 75-83.
Fadel, S, and Sarzotti, M. "Cellular immune responses in neonates." Int Rev Immunol 19, no. 2-3 (2000): 173-193. (Review)