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Mari L. Shinohara

Associate Professor of Immunology
Campus Mail: 338JONES Building, 207 Researc, Box 3010 Dumc, Dept of Immunology, Durham, NC 27710
Phone: (919) 613-6977
Email: mari.shinohara@duke.edu

Shinohara Lab Website

Immune responses against pathogens are essential for host protection, but excessive and uncontrolled immune reactions can lead to autoimmunity. How does our immune system keep the balance fine-tuned? This is a central question being asked in my laboratory.

The immune system needs to detect pathogens quickly and effectively. This is performed by the innate immune system, which includes cells such as macrophages and dendritic cells (DCs). Pathogens are recognized by pattern recognition receptors (PRRs) and may be cleared in the innate immune system. However, when pathogens cannot be eliminated by innate immunity, the adaptive immune system participates by exploiting the ability of T cells and B cells. The two immune systems work together not only to clear pathogens effectively but also to avoid collateral damages by our own immune responses. 

In my lab, we use mouse models for infectious and autoimmune diseases to understand the cellular and molecular mechanisms of; pathogen recognition by PRRs in macrophages and DCs, initiation of inflammatory responses in the innate immune system, and the impact of innate immune inflammation on the development and regulation of T cell-mediated adaptive immune responses. 

Several projects are ongoing in the lab. They are to study (1) the roles of PRR in EAE (an animal model of multiple sclerosis), (2) the interplay between immune cells and CNS (central nervous system)-resident cells during EAE and fungal infection, (3) protective and pathogenic mechanisms of immune cells in the lung during fungal infection and inflammation, and (4) the roles of a protein termed osteopontin (OPN), as both secreted (sOPN) and intracellular (iOPN) isoforms, in regulation of immune responses . Although we are very active in EAE to study autoimmunity, other mouse models, such as graft-versus-host disease (GvHD) is ongoing. Cell types we study are mainly DCs, macrophagesneutrophils, and T cells

Selected Publications

Liang, Jie, Junyi J. Zhang, Hsin-I Huang, Masashi Kanayama, Nourhan Youssef, Yingai J. Jin, Estefany Y. Reyes, et al. “The Ubiquitin-Modifying Enzyme A20 Terminates C-Type Lectin Receptor Signals and Is a Suppressor of Host Defense against Systemic Fungal Infection.” Infect Immun 88, no. 9 (August 19, 2020). https://doi.org/10.1128/IAI.00048-20.

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Ide, Shintaro, Yasuhito Yahara, Yoshihiko Kobayashi, Sarah A. Strausser, Kana Ide, Anisha Watwe, Shengjie Xu-Vanpala, et al. “Yolk-sac-derived macrophages progressively expand in the mouse kidney with age.” Elife 9 (April 17, 2020). https://doi.org/10.7554/eLife.51756.

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Yahara, Yasuhito, Tomasa Barrientos, Yuning J. Tang, Vijitha Puviindran, Puviindran Nadesan, Hongyuan Zhang, Jason R. Gibson, et al. “Erythromyeloid progenitors give rise to a population of osteoclasts that contribute to bone homeostasis and repair.” Nat Cell Biol 22, no. 1 (January 2020): 49–59. https://doi.org/10.1038/s41556-019-0437-8.

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Kanayama, Masashi, Shengjie Xu, Keiko Danzaki, Jason R. Gibson, Makoto Inoue, Simon G. Gregory, and Mari L. Shinohara. “Skewing of the population balance of lymphoid and myeloid cells by secreted and intracellular osteopontin.” Nat Immunol 18, no. 9 (September 2017): 973–84. https://doi.org/10.1038/ni.3791.

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Singh, Nina, Makoto Inoue, Ryosuke Osawa, Marilyn M. Wagener, and Mari L. Shinohara. “Inflammasome expression and cytomegalovirus viremia in critically ill patients with sepsis.” J Clin Virol 93 (August 2017): 8–14. https://doi.org/10.1016/j.jcv.2017.05.012.

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Schmidt, Elyse A., Brian E. Fee, Stanley C. Henry, Amanda G. Nichols, Mari L. Shinohara, Jeffrey C. Rathmell, Nancie J. MacIver, et al. “Metabolic Alterations Contribute to Enhanced Inflammatory Cytokine Production in Irgm1-deficient Macrophages.” J Biol Chem 292, no. 11 (March 17, 2017): 4651–62. https://doi.org/10.1074/jbc.M116.770735.

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Barclay, William, and Mari L. Shinohara. “Inflammasome activation in multiple sclerosis and experimental autoimmune encephalomyelitis (EAE).” Brain Pathol 27, no. 2 (March 2017): 213–19. https://doi.org/10.1111/bpa.12477.

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Inoue, Makoto, and Mari L. Shinohara. “Cutting edge: Role of osteopontin and integrin αv in T cell-mediated anti-inflammatory responses in endotoxemia.” J Immunol 194, no. 12 (June 15, 2015): 5595–98. https://doi.org/10.4049/jimmunol.1500623.

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Kanayama, Masashi, You-Wen He, and Mari L. Shinohara. “The lung is protected from spontaneous inflammation by autophagy in myeloid cells.” J Immunol 194, no. 11 (June 1, 2015): 5465–71. https://doi.org/10.4049/jimmunol.1403249.

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Kanayama, Masashi, Makoto Inoue, Keiko Danzaki, Gianna Hammer, You-Wen He, and Mari L. Shinohara. “Autophagy enhances NFκB activity in specific tissue macrophages by sequestering A20 to boost antifungal immunity.” Nat Commun 6 (January 22, 2015): 5779. https://doi.org/10.1038/ncomms6779.

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Gerriets, Valerie A., Rigel J. Kishton, Amanda G. Nichols, Andrew N. Macintyre, Makoto Inoue, Olga Ilkayeva, Peter S. Winter, et al. “Metabolic programming and PDHK1 control CD4+ T cell subsets and inflammation.” J Clin Invest 125, no. 1 (January 2015): 194–207. https://doi.org/10.1172/JCI76012.

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Inoue, Makoto, Tomohiro Arikawa, Yu-Hsun Chen, Yasuhiro Moriwaki, Michael Price, Michael Brown, John R. Perfect, and Mari L. Shinohara. “T cells down-regulate macrophage TNF production by IRAK1-mediated IL-10 expression and control innate hyperinflammation.” Proc Natl Acad Sci U S A 111, no. 14 (April 8, 2014): 5295–5300. https://doi.org/10.1073/pnas.1321427111.

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Wang, Bin, Yan-Hua Rao, Makoto Inoue, Rui Hao, Chun-Hsiang Lai, David Chen, Stacey L. McDonald, et al. “Microtubule acetylation amplifies p38 kinase signalling and anti-inflammatory IL-10 production.” Nat Commun 5 (March 17, 2014): 3479. https://doi.org/10.1038/ncomms4479.

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