Kanayama, M, Xu, S, Danzaki, K, Gibson, JR, Inoue, M, Gregory, SG, and Shinohara, ML. "Skewing of the population balance of lymphoid and myeloid cells by secreted and intracellular osteopontin." Nature Immunology 18, no. 9 (September 2017): 973-984.
Mari L. Shinohara
We need to mount a strong immune response against pathogens during infections, but excessive and uncontrolled immune reactions can lead to autoimmunity. How does our immune system keep the balance fine-tuned? This is a central question being asked in my laboratory.
Immune system needs to detect pathogens quickly and effectively. This is performed by the innate immune system, which includes cells such as macrophages and dendritic cells (DCs). Pathogens are recognized by pattern recognition receptors (PRRs) and may be cleared in the innate immune system. However, when pathogens cannot be eliminated by innate immunity, the adaptive immune system participates by exploiting the ability of T cells and B cells. The two immune systems work together not only to clear pathogens effectively but also to avoid collateral damages by from our own immune responses.
In my lab, we use mouse models for infectious and autoimmune diseases to understand the cellular and molecular mechanisms of; pathogen recognition by PRRs in macrophages and DCs, initiation of inflammatory responses in the innate immune system, and the impact of innate immune inflammation on the development and regulation of T cell-mediated adaptive immune responses.
Several projects are ongoing in the lab. They are; (1) elucidating the role of the NLRP3 inflammasome, an innate immune sensor of pathogens and endogenous danger signals, in T-cell mediated pathology of EAE (an animal model of multiple sclerosis), (2) dissecting molecular mechanisms of pathogen recognition through Toll-like receptors (TLRs) and c-type lectin receptors (CLRs) and of downregulating hyperinflammation, (3) molecular and cellular mechanisms in the innate immune system to induce immune tolerance in T cells, and (4) elucidating a role of a protein termed osteopontin (OPN), as both secreted (sOPN) and intracellular (iOPN) isoforms, in regulation of immune responses during infections and tumor development. Although we are very active in EAE to study autoimmunity, other mouse models, such as psoriasis and colitis are ongoing. As for infections, we are interested in fungal infections, which have not been well explored as bacterial and viral infections. Cell types we study are mainly DCs, macrophages, and T cells. By focusing on these immune cell types, we study impacts of infections on the development of autoimmunity.
Barclay, W, and Shinohara, ML. "Inflammasome activation in multiple sclerosis and experimental autoimmune encephalomyelitis (EAE)." Brain Pathology (Zurich, Switzerland) 27, no. 2 (March 2017): 213-219. (Review)
Inoue, M, Chen, P-H, Siecinski, S, Li, Q-J, Liu, C, Steinman, L, Gregory, SG, Benner, E, and Shinohara, ML. "An interferon-β-resistant and NLRP3 inflammasome-independent subtype of EAE with neuronal damage." Nature Neuroscience 19, no. 12 (December 2016): 1599-1609.
Danzaki, K, Kanayama, M, Alcazar, O, and Shinohara, ML. "Osteopontin has a protective role in prostate tumor development in mice." European journal of immunology 46, no. 11 (November 2016): 2669-2678.
Kanayama, M, Danzaki, K, He, Y-W, and Shinohara, ML. "Lung inflammation stalls Th17-cell migration en route to the central nervous system during the development of experimental autoimmune encephalomyelitis." International Immunology 28, no. 9 (September 2016): 463-469.
Kanayama, M, and Shinohara, ML. "Roles of Autophagy and Autophagy-Related Proteins in Antifungal Immunity." Frontiers in Immunology 7 (January 2016): 47-null. (Review)
Inoue, M, and Shinohara, ML. "Hyperinflammation, T cells, and endotoxemia." Oncotarget 6, no. 27 (September 2015): 23040-23041.
Inoue, M, and Shinohara, ML. "Cutting edge: Role of osteopontin and integrin αv in T cell-mediated anti-inflammatory responses in endotoxemia." Journal of immunology (Baltimore, Md. : 1950) 194, no. 12 (June 2015): 5595-5598.
Kanayama, M, He, Y-W, and Shinohara, ML. "The lung is protected from spontaneous inflammation by autophagy in myeloid cells." Journal of immunology (Baltimore, Md. : 1950) 194, no. 11 (June 2015): 5465-5471.
Kanayama, M, Inoue, M, Danzaki, K, Hammer, G, He, Y-W, and Shinohara, ML. "Autophagy enhances NFκB activity in specific tissue macrophages by sequestering A20 to boost antifungal immunity." Nature Communications 6 (January 22, 2015): 5779-null.
Gerriets, VA, Kishton, RJ, Nichols, AG, Macintyre, AN, Inoue, M, Ilkayeva, O, Winter, PS, Liu, X, Priyadharshini, B, Slawinska, ME, Haeberli, L, Huck, C, Turka, LA, Wood, KC, Hale, LP, Smith, PA, Schneider, MA, MacIver, NJ, Locasale, JW, Newgard, CB, Shinohara, ML, and Rathmell, JC. "Metabolic programming and PDHK1 control CD4+ T cell subsets and inflammation." The Journal of Clinical Investigation 125, no. 1 (January 2015): 194-207.
Inoue, M, Arikawa, T, Chen, Y-H, Moriwaki, Y, Price, M, Brown, M, Perfect, JR, and Shinohara, ML. "T cells down-regulate macrophage TNF production by IRAK1-mediated IL-10 expression and control innate hyperinflammation." Proceedings of the National Academy of Sciences of the United States of America 111, no. 14 (April 2014): 5295-5300.
Wang, B, Rao, Y-H, Inoue, M, Hao, R, Lai, C-H, Chen, D, McDonald, SL, Choi, M-C, Wang, Q, Shinohara, ML, and Yao, T-P. "Microtubule acetylation amplifies p38 kinase signalling and anti-inflammatory IL-10 production." Nature Communications 5 (March 17, 2014): 3479-null.
Inoue, M, and Shinohara, ML. "Clustering of pattern recognition receptors for fungal detection." Plos Pathogens 10, no. 2 (February 20, 2014): e1003873-null.
Inoue, M, and Shinohara, ML. "The role of interferon-β in the treatment of multiple sclerosis and experimental autoimmune encephalomyelitis - in the perspective of inflammasomes." Immunology 139, no. 1 (May 2013): 11-18. (Review)