Cui, Jing, Yi Ding, Shu Chen, Xiaoqiang Zhu, Yichen Wu, Mingliang Zhang, Yaxin Zhao, et al. “Disruption of Gpr45 causes reduced hypothalamic POMC expression and obesity..” J Clin Invest 126, no. 9 (September 1, 2016): 3192–3206. https://doi.org/10.1172/JCI85676.
Yuan Zhuang, PhD
Professor of Immunology
Campus Mail:
328 Jones Building, 207 Resear, Box 3010 DUMC, Durham, NC 27710
Phone:
(919) 613-7824
Email:
yzhuang@duke.edu
Research in our laboratory focuses on molecular mechanism of lymphocyte development and genetic basis of lymphoid diseases such as leukemia and autoimmune disorders. We are using mouse models to investigate the role of E2A and other related transcription factors in lymphocyte development and immune function. We have shown that E2A plays an essential role in regulating both B and T cell development. Mice deficient for E2A cannot produce any B cell and exhibit high incidence of T cell leukemia. Separate studies also indicated that E2A is involved in the development of autoimmune disorders. Our current and future studies are to combine genetic, molecular biology, and immunology means to determine gene expression programs during lymphocyte development.
The mechanistic studies of gene function in animal models provide important clues in understanding relevant human diseases. It has been shown that about 20-30% of pediatric acute lymphocytic leukemias (ALL) are linked to chromosomal rearrangements at the E2A gene locus. Most of these genetic defects produce oncogenic forms of E2A proteins, which are the possible cause of leukemia. The use of animal models allows us to further define the molecular events underlying the disease development. Our long term goal is to provide molecular basis for early diagnosis and treatment of relevant lymphoid system diseases.
Laboratory website: Http://sites.duke.edu/zhuanglab.
Education and Training
- Ph.D., Yale University, 1989
Selected Grants and Awards
- E2A/HEB MEDIATED TRANSCRIPTIONAL REGULATION IN T CELL DEVELOPMENT
- E2A/HEB MEDIATED TRANSCRIPTIONAL REGULATION IN T CELL DEVELOPMENT
- Organization and Function of Cellular Structure
- Organization and Function of Cellular Structure
- A New Method for Mosaic Analysis of Clonal Mutations in Mice
- Genetic dissection of Id3-mediated pathways in lineage development
- Basic Immunology Training Program
- Training Program in Inflammatory and Immunological Diseases
- A new approach to homeostatic maintenance of dendritic epidermal T cells
- Cancer Biology Training Grant
- A New Genetic Tool for Lineage Tracing of Mitotic Cells in Mice
- An episomal marking system for tracking cell proliferation
- Functions of E2A in B Lymphocyte Development
- Modeling Sjogrens Syndrome with Id3 Conditional Knockout Mice
- Orphan Nuclear Receptor in Thymocyte Differentiation
- The Functions Of E2a In B-Lymphocyte Development
- Cancer Center Core Support Grant
- Comprehensive Cancer Center Core Support Grant
Selected Publications
Zhang, Baojun, Qingzhu Jia, Cheryl Bock, Gang Chen, Haili Yu, Qingshan Ni, Ying Wan, Qijing Li, and Yuan Zhuang. “Glimpse of natural selection of long-lived T-cell clones in healthy life..” Proc Natl Acad Sci U S A 113, no. 35 (August 30, 2016): 9858–63. https://doi.org/10.1073/pnas.1601634113.
Ye, Zhisheng, Lei Sun, Rongbo Li, Min Han, Yuan Zhuang, Xiaohui Wu, and Tian Xu. “Generation of a Mouse Full-length Balancer with Versatile Cassette-shuttling Selection Strategy..” Int J Biol Sci 12, no. 8 (2016): 911–16. https://doi.org/10.7150/ijbs.15172.
Zhang, Baojun, Jianxuan Wu, Yiqun Jiao, Cheryl Bock, Meifang Dai, Benny Chen, Nelson Chao, Weiguo Zhang, and Yuan Zhuang. “Differential Requirements of TCR Signaling in Homeostatic Maintenance and Function of Dendritic Epidermal T Cells..” J Immunol 195, no. 9 (November 1, 2015): 4282–91. https://doi.org/10.4049/jimmunol.1501220.
Ye, Jian, Hao Shi, Ye Shen, Chao Peng, Yan Liu, Chenyu Li, Kejing Deng, et al. “PP6 controls T cell development and homeostasis by negatively regulating distal TCR signaling..” J Immunol 194, no. 4 (February 15, 2015): 1654–64. https://doi.org/10.4049/jimmunol.1401692.
Roy, Sumedha, and Yuan Zhuang. “Orchestration of invariant natural killer T cell development by E and Id proteins..” Crit Rev Immunol 35, no. 1 (2015): 33–48.
Belle, Ian, Josh Mahlios, Andrew McKenzie, and Yuan Zhuang. “Aberrant production of IL-13 by T cells promotes exocrinopathy in Id3 knockout mice..” Cytokine 69, no. 2 (October 2014): 226–33. https://doi.org/10.1016/j.cyto.2014.06.009.
Niola, F., X. Zhao, D. Singh, R. Sullivan, A. Castano, A. Verrico, P. Zoppoli, et al. “Mesenchymal high-grade glioma is maintained by the ID-RAP1 axis..” J Clin Invest 124, no. 9 (September 2, 2014). https://doi.org/10.1172/JCI77927.
Zhang, Baojun, Yen-Yu Lin, Meifang Dai, and Yuan Zhuang. “Id3 and Id2 act as a dual safety mechanism in regulating the development and population size of innate-like γδ T cells..” J Immunol 192, no. 3 (February 1, 2014): 1055–63. https://doi.org/10.4049/jimmunol.1302694.
Li, Jia, Di Wu, Ning Jiang, and Yuan Zhuang. “Combined deletion of Id2 and Id3 genes reveals multiple roles for E proteins in invariant NKT cell development and expansion..” J Immunol 191, no. 10 (November 15, 2013): 5052–64. https://doi.org/10.4049/jimmunol.1301252.
Zhang, Baojun, Meifang Dai, Qi-Jing Li, and Yuan Zhuang. “Tracking proliferative history in lymphocyte development with cre-mediated sister chromatid recombination..” Plos Genet 9, no. 10 (October 2013). https://doi.org/10.1371/journal.pgen.1003887.
Lin, Yen-Yu, Mary E. Jones-Mason, Makoto Inoue, Anna Lasorella, Antonio Iavarone, Qi-Jing Li, Mari L. Shinohara, and Yuan Zhuang. “Transcriptional regulator Id2 is required for the CD4 T cell immune response in the development of experimental autoimmune encephalomyelitis..” J Immunol 189, no. 3 (August 1, 2012): 1400–1405. https://doi.org/10.4049/jimmunol.1200491.
Zhu, Yi, Shiwei Liu, Qili Yin, Tian Xu, Xiaohui Wu, and Yuan Zhuang. “Generation of Dhx9-deficient clones in T-cell development with a mitotic recombination technique..” Genesis 50, no. 7 (July 2012): 543–51.
Jones-Mason, Mary Elizabeth, Xudong Zhao, Dietmar Kappes, Anna Lasorella, Antonio Iavarone, and Yuan Zhuang. “E protein transcription factors are required for the development of CD4(+) lineage T cells..” Immunity 36, no. 3 (March 23, 2012): 348–61. https://doi.org/10.1016/j.immuni.2012.02.010.
Mahlios, Josh, and Yuan Zhuang. “Contribution of IL-13 to early exocrinopathy in Id3-/- mice..” Mol Immunol 49, no. 1–2 (October 2011): 227–33. https://doi.org/10.1016/j.molimm.2011.08.012.